Journal: Journal of Translational Medicine

Article Title: Astrocyte elevated gene-1 (AEG-1) is a marker for aggressive salivary gland carcinoma

doi: 10.1186/1479-5876-9-205

Figure Lengend Snippet: Upregulation of AEG-1 expression in SGC tissues . (A and B) Expression of AEG-1 protein and mRNA in 2 normal human salivary gland tissues and 8 SGC tissues by Western blot (A) and real-time PCR (B), respectively. * P < 0.05, ** P < 0.01, *** P < 0.001. (C) Expression of AEG-1 protein in each of the primary SGC tissues (T) and adjacent non-cancerous tissues (N) in the same patient determined by Western blot. (D) Real time-PCR analysis of AEG-1 expression in each of the T and N tissues. GAPDH was used as an internal control. Columns, mean from three parallel experiments; bars, SD. (E) Expression of AEG-1 mRNA in each of the primary SGC tissues (lower panel) and adjacent non-cancerous tissues (upper panel) paired from the same patient determined by IHC.

Article Snippet: Western blots were performed according to standard methods as described previously [ ], using a rabbit anti-AEG-1 polyclonal antibody (1:500; Zymed).

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction

Journal: Journal of Translational Medicine

Article Title: Astrocyte elevated gene-1 (AEG-1) is a marker for aggressive salivary gland carcinoma

doi: 10.1186/1479-5876-9-205

Figure Lengend Snippet: AEG-1 protein overexpression in archived paraffin-embedded SGC tissue sections as examined by IHC . (A) Representative images from IHC analyses of AEG-1 expression in seven histological types of SGC. (B) Representative images from IHC analyses of AEG-1 expression in normal human salivary gland tissues and primary SGC specimens. (C) Statistical analyses of the average MOD of AEG-1 staining between normal salivary gland tissues (2 cases) and SGC specimens of different clinical stages. * P < 0.05.

Article Snippet: Western blots were performed according to standard methods as described previously [ ], using a rabbit anti-AEG-1 polyclonal antibody (1:500; Zymed).

Techniques: Over Expression, Expressing, Staining

Journal: Journal of Translational Medicine

Article Title: Astrocyte elevated gene-1 (AEG-1) is a marker for aggressive salivary gland carcinoma

doi: 10.1186/1479-5876-9-205

Figure Lengend Snippet: Kaplan-Meier curves with univariate analyses (log-rank) for patients with low AEG-1 expression (bold line) versus high AEG-1 expression tumors (dotted line) . The cumulative 5-year survival rate was 78.4% in the low AEG-1 protein expression group (n = 62), whereas it was only 45.0% in the high AEG-1 expression group (n = 79).

Article Snippet: Western blots were performed according to standard methods as described previously [ ], using a rabbit anti-AEG-1 polyclonal antibody (1:500; Zymed).

Techniques: Expressing

Journal: Journal of Translational Medicine

Article Title: Astrocyte elevated gene-1 (AEG-1) is a marker for aggressive salivary gland carcinoma

doi: 10.1186/1479-5876-9-205

Figure Lengend Snippet: Overall survival curves stratified by AEG-1 levels according to clinical stage, T and M classifications . (A, B) The differences of survival curves according to AEG-1 expression were seen in advanced clinical stage (B), but not in early clinical stage (A). (C, D) In the T3-T4 subgroup, patients with low AEG-1 expression showed significantly better overall survival (D). In the T1-T2 subgroup, the patients' overall survival times were not significantly different between the two groups (C). (E, F) Survival time was longer in patients with low AEG-1 expression regardless of distant metastasis (F). No significant difference between low and high AEG-1 expression groups were found in the M0 subgroup (E).

Article Snippet: Western blots were performed according to standard methods as described previously [ ], using a rabbit anti-AEG-1 polyclonal antibody (1:500; Zymed).

Techniques: Expressing

Journal: BMC complementary medicine and therapies

Article Title: Overcoming cisplatin resistance by targeting the MTDH-PTEN interaction in ovarian cancer with sera derived from rats exposed to Guizhi Fuling wan extract.

doi: 10.1186/s12906-020-2825-9

Figure Lengend Snippet: Fig. 2 Protein Ligand interaction with LY294002 and 3-dehydrotrametenolic acid. The systemsDock website was used to predict the possible PTEN/MTDH protein ligand interaction with the compounds. a. PTEN protein ligands with 3-dehydrotrametenolic acid, b. MTDH protein ligands with 3-dehydrotrametenolic acid, c. PTEN protein ligands with LY294002, d. MTDH protein ligands with LY294002

Article Snippet: The membranes were then incubated with a rabbit polyclonal antibody against MTDH (1:600 dilution; Proteintech Group, China), a mouse monoclonal antibody against PTEN (1: 1000 dilution; Proteintech Group, China), and a mouse monoclonal antibody against GAPDH (1:4000 dilution; Proteintech Group, China) at 4 °C overnight.

Techniques:

Journal: BMC complementary medicine and therapies

Article Title: Overcoming cisplatin resistance by targeting the MTDH-PTEN interaction in ovarian cancer with sera derived from rats exposed to Guizhi Fuling wan extract.

doi: 10.1186/s12906-020-2825-9

Figure Lengend Snippet: Fig. 3 Relative quantification of MTDH and PTEN mRNA in SKOV3/DDP cells. A 2−ΔΔCt method was used to analyze the mRNA expression changes after treatment with sera containing GFWE or LY294002, and GAPDH was used as an internal standard. LD sera = low-dose sera containing GFWE, MD sera = middle-dose sera containing GFWE, HD sera = high-dose sera containing GFWE. Error bars represent the SD. *P < 0.05, **P < 0.01

Article Snippet: The membranes were then incubated with a rabbit polyclonal antibody against MTDH (1:600 dilution; Proteintech Group, China), a mouse monoclonal antibody against PTEN (1: 1000 dilution; Proteintech Group, China), and a mouse monoclonal antibody against GAPDH (1:4000 dilution; Proteintech Group, China) at 4 °C overnight.

Techniques: Quantitative Proteomics, Expressing

Journal: BMC complementary medicine and therapies

Article Title: Overcoming cisplatin resistance by targeting the MTDH-PTEN interaction in ovarian cancer with sera derived from rats exposed to Guizhi Fuling wan extract.

doi: 10.1186/s12906-020-2825-9

Figure Lengend Snippet: Fig. 4 The protein expression changes of MTDH and PTEN in SKOV3/DDP cells treated with the sera containing GFWE. (a) Representative images for MTDH and PTEN investigated. All images were obtained using a 40× Plan-Apochromat objective of the LSM800 confocal microscope. The nuclei were counterstained with DAPI (blue). 400× magnification, bar = 20 μm. (b) The MFIs of MTDH and PTEN in the cells measured with the graphics module of Zeiss Zen software. Error bars represent the SD. (c) Western blotting analyses demonstrated a strong decrease in the levels of MTDH protein and a strong increase in the levels of PTEN in SKOV3/DDP cells by the sera or LY294002. The mAb-PTEN, pAb-MTDH and mAb- GAPDH were used for blotting. A total of 20 μg of protein per sample was loaded to each lane. * P < 0.5, **P < 0.01. NS = No significant difference

Article Snippet: The membranes were then incubated with a rabbit polyclonal antibody against MTDH (1:600 dilution; Proteintech Group, China), a mouse monoclonal antibody against PTEN (1: 1000 dilution; Proteintech Group, China), and a mouse monoclonal antibody against GAPDH (1:4000 dilution; Proteintech Group, China) at 4 °C overnight.

Techniques: Expressing, Microscopy, Software, Western Blot

Journal: BMC complementary medicine and therapies

Article Title: Overcoming cisplatin resistance by targeting the MTDH-PTEN interaction in ovarian cancer with sera derived from rats exposed to Guizhi Fuling wan extract.

doi: 10.1186/s12906-020-2825-9

Figure Lengend Snippet: Fig. 5 Representative images show MTDH colocalization with PTEN. All images were obtained using the 63× Plan-Apochromat oil objective of the LSM800 confocal microscope; 630× magnification, bar = 10 μm. All of the images are merged pictures of the green-colored Alexa Fluor-488 tagged the PTEN protein, the red-colored Alexa Fluor-647-tagged the MTDH protein, and the blue was the nucleus counterstained by DAPI

Article Snippet: The membranes were then incubated with a rabbit polyclonal antibody against MTDH (1:600 dilution; Proteintech Group, China), a mouse monoclonal antibody against PTEN (1: 1000 dilution; Proteintech Group, China), and a mouse monoclonal antibody against GAPDH (1:4000 dilution; Proteintech Group, China) at 4 °C overnight.

Techniques: Microscopy

Journal: BMC complementary medicine and therapies

Article Title: Overcoming cisplatin resistance by targeting the MTDH-PTEN interaction in ovarian cancer with sera derived from rats exposed to Guizhi Fuling wan extract.

doi: 10.1186/s12906-020-2825-9

Figure Lengend Snippet: Fig. 6 Degree of MTDH colocalization with PTEN in SKOV3/DDP cells. This bar graph represents the corresponding mean Pearson’s coefficient and Mander’s coefficient for MTDH and PTEN affected by the sera containing GFWE and LY294002. Error bars represent the SD. All calculations for Pearson’s and Mander’s coefficient have been analyzed by the confocal topography module of Zeiss Zen software. ***P < 0.001. NS = No significant difference

Article Snippet: The membranes were then incubated with a rabbit polyclonal antibody against MTDH (1:600 dilution; Proteintech Group, China), a mouse monoclonal antibody against PTEN (1: 1000 dilution; Proteintech Group, China), and a mouse monoclonal antibody against GAPDH (1:4000 dilution; Proteintech Group, China) at 4 °C overnight.

Techniques: Software

Journal: BMC complementary medicine and therapies

Article Title: Overcoming cisplatin resistance by targeting the MTDH-PTEN interaction in ovarian cancer with sera derived from rats exposed to Guizhi Fuling wan extract.

doi: 10.1186/s12906-020-2825-9

Figure Lengend Snippet: Fig. 7 Identification and characterization of the MTDH-PTEN interaction. (a) Analysis of the MTDH-PTEN interaction by the STRING database. The edge indicates an interaction between the two proteins. (b) Interaction of PTEN with MTDH detected by co-IP. The pAb-MTDH was used for immunoprecipitation, and a rabbit IgG pAb was used as a control antibody. The mAb-PTEN was used for western blotting. The Input blot of PTEN indicates non-SureBeads-handled cell lysate to prove that there was the protein of interest in the sample to begin with

Article Snippet: The membranes were then incubated with a rabbit polyclonal antibody against MTDH (1:600 dilution; Proteintech Group, China), a mouse monoclonal antibody against PTEN (1: 1000 dilution; Proteintech Group, China), and a mouse monoclonal antibody against GAPDH (1:4000 dilution; Proteintech Group, China) at 4 °C overnight.

Techniques: Co-Immunoprecipitation Assay, Immunoprecipitation, Control, Western Blot

Journal: Molecular medicine reports

Article Title: Bruton's tyrosine kinase inhibitor restrains Wnt signaling in chronic lymphocytic leukemia.

doi: 10.3892/mmr.2016.5111

Figure Lengend Snippet: Figure 4. Downregulation of MTDH expression in MEC‑1 cells subsequent to ibrutinib treatment (*P<0.05). MTDH, metadherin.

Article Snippet: The antibodies used in the current study were as follows: Rabbit polyclonal anti-MTDH antibody (#13860-1-AP; 1:1,000; Proteintech Group, Inc., Chicago, IL, USA), rabbit monoclonal anti-β-catenin (#8480; 1:1,000; Cell Signaling Technology, Inc., Danvers, MA, USA), rabbit polyclonal anti-LEF-1 (#14972-1-AP; 1:1,000; Proteintech Group, Inc.), mouse anti-human monoclonal β-actin antibody (1:2,000) (#TA-09, OriGene Technologies, Inc., Beijing, China).

Techniques: Expressing